C-flex tubing ....................................................... 46
DeltaV control platform ..................................... 51
DO probe ............................................................ 51
extracellular matrix coating ................................ 43
F3 hPSC passaging solution.........................49, 52
harvest line extension.......................................... 42
L7™culture system................................ 40, 49, 50
microcarrier coating............................................ 44
passaging solution............................................... 40
Pharmed tubing portion..................................... 47
T-75 flask............................................................. 43
tubing ............................................................40–41
2D seed train .................................................43–44
using peristaltic pump......................................... 52
Suspension bioreactors .......................194–197, 199, 202
T
Tendon-derived stem cells (TDSCs)
definition .................................................................135
isolation of mice TDSCs................................ 137–139
mechanical simulation
extraction of RNA for validation ....................138,
141–142
scaffold-based ...................................137, 140–141
scaffold-free ......................................137, 139–140
primer sequences for RT-qPCR analysis................142
scaffold-based method............................................136
tenogenesis marker..................................................136
Tenogenesis, of TDSCs ................................................136
Thermoplastic microfluidic devices, fabrication protocol
biological and medical applications.......................... 27
chip design and preparation
electrochemical wet etching ............................... 32
photolithography ..........................................29–31
using AutoCAD software ................................... 31
drilling and planarization.......................................... 34
of electrodes
blank COP substrate.............................. 32–33, 36
Cr/Au etching .................................................... 33
design creation .................................................... 32
hot embossing .....................................................34–36
market and market reports ....................................... 28
materials..................................................................... 29
microfluidic device with electrodes .......................... 36
microfluidic device without electrodes ..............35–36
modification and durability ...................................... 28
optical properties....................................................... 28
thermo-compression bonding.................................. 35
3D scaffolds, cell activity in
bioartificial tissues ...................................................127
bioreactor system ........................................... 129–130
mechanical compression load and fluid flow
parameters....................................................131
sample preparation ..................................................130
sample transfer and chamber assembly ..................131
3D TableTrix®...............................................................114
Three-dimensional (3D) tissue equivalents
epithelial polarization..............................................246
fluid flow..................................................................242
liver model...............................................................245
materials
cell culture ......................................................... 248
processing and analysis of samples ................... 249
methods
drug toxicity testing, HepG2 liver model growth
for.........................................................249–251
perfused intestinal model creation ...........251–253
perfused model, processing and analysis
of ..........................................................253–255
static intestine models.............................................247
techniques................................................................243
transport ..................................................................242
Tissue engineering ...................................... 127, 167, 180
Tracheal graft de-epithelialization
antibiotics, media, and detergents
cocktail of antibiotics ........................................ 171
DMEM + antibiotics.................................171–172
materials.....................................................170–171
1% sodium dodecyl sulfate................................ 172
1% triton X-100................................................. 172
bioreactor extra components......................... 168–169
bioreactor main components......................... 168, 169
bioreactor setup.............................................. 175–176
decontamination ............................................ 179–180
detergent circuitries ....................................... 173–174
devices......................................................................170
graft procurement and preparation........................172
H2O ................................................................ 177, 178
motor and pumps installation ....................... 176–177
other materials................................................ 169–170
PBS...........................................................................179
positioning trachea..................................................176
SDS ..........................................................................177
tools .........................................................................170
triton X-100 ................................................... 177, 178
Tracheal in vitro reconstruction.......................... 157–160
bioreactor.................................................................165
materials
generation, decellularized tracheal ECM-based
porcine bio-scaffold.............................160–161
human chondrocyte propagation and
maintenance................................................. 161
porcine trachea collection................................. 160
repopulation, decellularized tracheal ECM-based
porcine bio-scaffold.............................161–162
methods
generation, decellularized tracheal ECM-based
porcine bio-scaffold.............................162–163
272 BIOREACTORS IN STEM CELL BIOLOGY: METHODS AND PROTOCOLS
Index